39.11:
Stem Cell Culture
Stem cell culture is the process of growing stem cells in vitro under optimal conditions.
Stem cells have a wide range of medical applications like tissue and organ regeneration.
Stem cell samples are usually extracted from adult or embryonic tissues.
These samples are then transferred to plastic culture dishes coated with a feeder layer consisting of inactivated mouse embryonic fibroblast cells, which provide necessary intercellular interactions and nutrients; and prevent the spontaneous differentiation of stem cells.
The culture dish is supplemented with media primarily containing growth hormones and essential nutrients. It nourishes stem cells to divide and spread over the culture dish, forming colonies.
Once grown to the required size, these colonies are removed gently and subjected to the passage, where they are further replated or subcultured multiple times to establish a pure and continuously growing cell line.
An established cell line can give rise to millions of stem cells in vitro. So, a batch of these cultured stem cells can be frozen for future use.
39.11:
Stem Cell Culture
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and repair body tissues.
The mechanisms that induce a non-differentiated cell to become a specialized cell are poorly understood. In a laboratory setting, it is possible to induce stem cells to differentiate into specialized cells by changing the physical and chemical conditions of growth. Several sources of stem cells are used experimentally and are classified according to their origin and potential for differentiation. Human embryonic stem cells (hESCs) are extracted from embryos and are pluripotent. The adult stem cells that are present in many organs and differentiated tissues, such as bone marrow and skin, are multipotent, being limited in differentiation to the types of cells found in those tissues.
The stem cells isolated from umbilical cord blood are also multipotent, as are cells from deciduous teeth (baby teeth). Researchers have recently developed induced pluripotent stem cells (iPSCs) from mouse and human adult stem cells. These cells are genetically reprogrammed multipotent adult cells that function like embryonic stem cells; they are capable of generating cells characteristic of all three germ layers.
Because of their capacity to divide and differentiate into specialized cells, stem cells offer a potential treatment for diseases such as diabetes and heart disease. Cell-based therapy refers to treatment in which stem cells induced to differentiate in a growth dish are injected into a patient to repair damaged or destroyed cells or tissues. Many obstacles must be overcome for the application of cell-based therapy. Although embryonic stem cells have a nearly unlimited range of differentiation potential, they are seen as foreign by the patient’s immune system and may trigger rejection. Also, the destruction of embryos to isolate embryonic stem cells raises considerable ethical and legal questions.
In contrast, adult stem cells isolated from a patient are not seen as foreign by the body, but they have a limited range of differentiation. Some individuals bank the cord blood or deciduous teeth of their child, storing away those sources of stem cells for future use, should their child need it. Induced pluripotent stem cells are considered a promising advance in the field because using them avoids the legal, ethical, and immunological pitfalls of embryonic stem cells.
This text is adapted from openstax Anatomy and physiology 2e, Section 3.6: Cell differentiation.