多普勒光学相干断层扫描视网膜循环
Summary
总的测量视网膜血流多普勒光学相干断层扫描和半自动化分级软件。
Abstract
傅立叶域光学相干断层扫描（OCT）系统使用一个circumpapillary双循环扫描（CDCS），它可以扫描周围的视神经头3.40毫米和3.75毫米直径的非接触式视网膜血流进行测量。双同心圆连续进行了6倍，超过2秒。 CDCS扫描保存与多普勒频移信息可以计算出从该流。标准的临床协议要求3 CDCS扫描的OCT光束通过的superonasal瞳孔边缘3 CDCS扫描通过inferonal瞳孔。这双角协议，确保可接受的每个视网膜分支血管多普勒角度上获得至少1次扫描。 CDCS扫描数据，一个3 – 维体积OCT扫描的视盘扫描，和视盘的彩色照片一起使用，以获得上的眼睛的视网膜血流测量。我们已经开发出一种血流量测量软件被称为“多普勒光纤人相干断层扫描视网膜循环“（DOCTORC）。此半自动化的软件被用来测量视网膜全血流量，血管的横截面面积，和平均血流速度，每个容器中的流是从容器截面的多普勒频移计算截面面积和多普勒角之间的容器中，在OCT束。共有视网膜血流量测量从视盘周围静脉相加得到的结果在我们的多普勒OCT读取中心平地机和方法（<10％之间表现出良好的再现性，OCT在青光眼患者视网膜血流测量高度与视野缺损（R 2> 0.57，视野模式偏差），总视网膜血流可能是有用的管理，青光眼，视网膜疾病，视网膜疾病。）多普勒OCT是一种新的方法来进行快速，非接触式和可重复的测量使用广泛可傅立叶域OCT instrumentatio的的总视网膜血流的。N。这项新技术可以改善的临床研究和常规临床实践中进行这些测量的实用性。
Protocol
1。协议文本患者被扫描RTVue傅立叶域光学相干断层成像（OCT）系统（公司Optovue，弗里蒙特，CA，美国）使用的circumpapillary的双圆形扫描（CDCS）和3D模型视盘扫描。 CDCS图案周围的视神经头由两个同心圆。内圈直径为3.40毫米，环的外直径为3.75毫米。这种模式横断所有的视网膜分支动脉和静脉产生的视神经头。双圈在单次扫描覆盖约2个心动周期的6倍。为了计算，多普勒频移和多普勒?…
Discussion
血流量异常发生在青光眼的视网膜血管性疾病，如糖 尿病性视网膜病变。6-10体积测量视网膜的血流量提供了有价值的信息4-6,11,12 DOCTORC提供了一个实用的方法来估计全视网膜疾病的进程。血流量的测量值的基础上使用的双圆圈扫描图案由多普勒OCT确定个别船只。1-5
平均总视网膜血流量测量多普勒OCT在正常的眼睛是47-49μl/ min的，与文献值34-65μl/ mi…
Disclosures
The authors have nothing to disclose.
Acknowledgements
这项研究是由美国国立卫生研究院授予RO1 013516和赠款形式Optovue的支持。
Materials
| Name of the reagent/equipment | Company | Catalogue number | Comments (optional) |
| RTVue Fourier Domain optical coherence tomography | Optovue | N/A | Version 6.1.0.21 or higher Installed with blood flow double ring scan pattern |
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Tags
Doppler Optical Coherence TomographyRetinal CirculationBlood Flow MeasurementsFourier Domain Optical Coherence TomographyCircumpapillary Double Circular ScanCDCS ScanDoppler Shift InformationFlow CalculationClinical ProtocolDouble-angle ProtocolRetinal Branch Vessel3-dimensional Volumetric OCT ScanColor Photograph Of The Optic DiscBlood Flow Measurement SoftwareDOCTORCTotal Retinal Blood FlowVessel Cross Section AreaAverage Blood Velocity
Cite This Article
Tan, O., Wang, Y., Konduru, R. K., Zhang, X., Sadda, S. R., Huang, D. Doppler Optical Coherence Tomography of Retinal Circulation. J. Vis. Exp. (67), e3524, doi:10.3791/3524 (2012).