Add 80 µl of 1 mM Pefa Bloc and 60 mg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).
to:
Add 80 µl of 1 mM Pefa Bloc and 60 µg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).
Step 5.2 in the Protocol was updated from:
Further dilute the Control Peptide from step 5.1 (20 mM) 1:100 into the MHC II Master Mix from step 3.2.
to:
Further dilute the Control Peptide from step 5.1 (20 µM) 1:100 into the MHC II Master Mix from step 3.2.
Add 80 µl of 1 mM Pefa Bloc and 60 mg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).
to:
Add 80 µl of 1 mM Pefa Bloc and 60 µg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).
Step 5.2 in the Protocol was updated from:
Further dilute the Control Peptide from step 5.1 (20 mM) 1:100 into the MHC II Master Mix from step 3.2.
to:
Further dilute the Control Peptide from step 5.1 (20 µM) 1:100 into the MHC II Master Mix from step 3.2.