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Errata

Erratum: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

doi: 10.3791/6309 Published: March 18, 2019

Abstract

An erratum was issued for: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes.  There were typos in the Protocol section and Table 2.

Step 3.1.1 in the Protocol was updated from:

Add 80 µl of 1 mM Pefa Bloc and 60 mg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).

to:

Add 80 µl of 1 mM Pefa Bloc and 60 µg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).

Step 5.2 in the Protocol was updated from:

Further dilute the Control Peptide from step 5.1 (20 mM) 1:100 into the MHC II Master Mix from step 3.2.

to:

Further dilute the Control Peptide from step 5.1 (20 µM) 1:100 into the MHC II Master Mix from step 3.2.

Table 2 was updated from:

MHC II Allele DRB1*: 1501
MHC II Stock Concentration (mg/ml) 1.3
MHC II Stock Concentration (mM) 20
Vol. MHC II Stock to Add (ml) 14.65
Vol. Reaction Buffer to Add (ml): 2885.35
MHC II Master Mix Concentration (nM) 101

to:

MHC II Allele DRB1*: 1501
MHC II Stock Concentration (mg/ml) 1.3
MHC II Stock Concentration (μM) 20
Vol. MHC II Stock to Add (μl) 14.65
Vol. Reaction Buffer to Add (μl): 2885.35
MHC II Master Mix Concentration (nM) 101

Protocol

An erratum was issued for: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes.  There were typos in the Protocol section and Table 2.

Step 3.1.1 in the Protocol was updated from:

Add 80 µl of 1 mM Pefa Bloc and 60 mg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).

to:

Add 80 µl of 1 mM Pefa Bloc and 60 µg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).

Step 5.2 in the Protocol was updated from:

Further dilute the Control Peptide from step 5.1 (20 mM) 1:100 into the MHC II Master Mix from step 3.2.

to:

Further dilute the Control Peptide from step 5.1 (20 µM) 1:100 into the MHC II Master Mix from step 3.2.

Table 2 was updated from:

MHC II Allele DRB1*: 1501
MHC II Stock Concentration (mg/ml) 1.3
MHC II Stock Concentration (mM) 20
Vol. MHC II Stock to Add (ml) 14.65
Vol. Reaction Buffer to Add (ml): 2885.35
MHC II Master Mix Concentration (nM) 101

to:

MHC II Allele DRB1*: 1501
MHC II Stock Concentration (mg/ml) 1.3
MHC II Stock Concentration (μM) 20
Vol. MHC II Stock to Add (μl) 14.65
Vol. Reaction Buffer to Add (μl): 2885.35
MHC II Master Mix Concentration (nM) 101

Disclosures

No conflicts of interest declared.

DOI

Cite this Article

Erratum: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes. J. Vis. Exp. (145), e6309, (2019).More

Erratum: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes. J. Vis. Exp. (145), e6309, (2019).

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