Erratum: A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

November 3rd, 2021

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Summary

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An erratum was issued for: A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice. The Authors section and a figure were updated.

Abstract

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An erratum was issued for: A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice. The Authors section and a figure were updated.

The authors section was updated from:

Sonia Simonetti*1,2, Ambra Natalini*1,2, Giovanna Peruzzi3, Alfredo Nicosia4, Antonella Folgori5, Stefania Capone5, Angela Santoni2, Francesca Di Rosa1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR),
2Department of Molecular Medicine, University of Rome “Sapienza”,
3Center for Life Nano Science, Istituto Italiano di Tecnologia,
4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II,
5Reithera Srl
* These authors contributed equally

To:

Sonia Simonetti*1,2, Ambra Natalini*1,2, Giovanna Peruzzi3, Alfredo Nicosia4, Antonella Folgori5, Stefania Capone5, Angela Santoni2,6, Francesca Di Rosa1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR),
2Department of Molecular Medicine, University of Rome “Sapienza”,
3Center for Life Nano Science, Istituto Italiano di Tecnologia,
4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II,
5Reithera Srl
6IRCCS, Neuromed
* These authors contributed equally

Figure 1 was updated from:

Lymph node, spleen, bone marrow flow cytometry workflow; staining, fixation, data analysis diagram.
Figure 1: Scheme of the protocol for cell cycle analysis of antigen-specific CD8 T cells. Please click here to view a larger version of this figure.

To:

Flow cytometry process diagram; lymph node and bone marrow cell staining, Ki67, DNA with Hoechst 33342.
Figure 1: Scheme of the protocol for cell cycle analysis of antigen-specific CD8 T cells. Please click here to view a larger version of this figure.

Protocol

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An erratum was issued for: A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice. The Authors section and a figure were updated.

The authors section was updated from:

Sonia Simonetti*1,2, Ambra Natalini*1,2, Giovanna Peruzzi3, Alfredo Nicosia4, Antonella Folgori5, Stefania Capone5, Angela Santoni2, Francesca Di Rosa1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR),
2Department of Molecular Medicine, University of Rome “Sapienza”,
3Center for Life Nano Science, Istituto Italiano di Tecnologia,
4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II,
5Reithera Srl
* These authors contributed equally

To:

Sonia Simonetti*1,2, Ambra Natalini*1,2, Giovanna Peruzzi3, Alfredo Nicosia4, Antonella Folgori5, Stefania Capone5, Angela Santoni2,6, Francesca Di Rosa1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR),
2Department of Molecular Medicine, University of Rome “Sapienza”,
3Center for Life Nano Science, Istituto Italiano di Tecnologia,
4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II,
5Reithera Srl
6IRCCS, Neuromed
* These authors contributed equally

Figure 1 was updated from:

Lymph node, spleen, bone marrow flow cytometry workflow; staining, fixation, data analysis diagram.
Figure 1: Scheme of the protocol for cell cycle analysis of antigen-specific CD8 T cells. Please click here to view a larger version of this figure.

To:

Flow cytometry process diagram; lymph node and bone marrow cell staining, Ki67, DNA with Hoechst 33342.
Figure 1: Scheme of the protocol for cell cycle analysis of antigen-specific CD8 T cells. Please click here to view a larger version of this figure.

Disclosures

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No conflicts of interest declared.

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Tags

DNA Ki67 AssayHoechst 33342 StainingLymph Node Spleen Bone MarrowProtocol Scheme DiagramCell Fixation MethodData Analysis Workflow