Cell Response Screening
Cell Response Screening - Related Videos
12:50
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
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Cited by 1 •
2017
Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.
15:31
MISSION esiRNA for RNAi Screening in Mammalian Cells
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Cited by 17 •
2010
Here we use a human esiRNA library in a high-throughput screen for genes involved in cell division. We demonstrate how to set up and conduct an esiRNA screens, as well as how to analyze and validate the results.
09:07
Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses
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Cited by 16 •
2017
Systemic and localized zebrafish infection models for human influenza A virus are demonstrated. Using a systemic infection model, zebrafish can be used to screen antiviral drugs. Using a localized infection model, zebrafish can be used to characterize host immune cell responses.
03:23
Cell Death Screening Using DAPI Staining: A Method for Rapid Screening of IR Induced Clonogenic Cell Death in Cancerous Cell Lines
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2023
This video demonstrates the methodology to screen for major cell death profiles in ionizing radiation (IR) induced cancer cell lines using DAPI staining assay. The DAPI-stained nuclei of target cells undergoing cell death exhibit distinct morphologies and can be easily identified by fluorescence microscopy.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
FAQs
Meaningful results depend on holding the tested conditions sufficiently defined while varying the stimulus or material feature of interest. Screens may examine biological, chemical, physical, or material-based inputs, then compare cellular changes across those conditions. This structure helps distinguish responses associated with the engineered environment from differences caused by uncontrolled treatment conditions.
These readouts capture different dimensions of cellular behavior rather than a single outcome. Viability, morphology, proliferation, gene expression, and signaling can each reveal a different change after exposure to a defined condition. Combining these measurements gives bioengineers a broader basis for judging whether a material, scaffold, drug, or engineered environment produces the intended cellular response.
Comparing treated cells with control conditions establishes whether an observed change is associated with the tested stimulus. Repeating comparisons across treatments also reveals how response varies as the engineered environment changes. This makes the screen useful for linking a specific design condition to cellular function rather than merely recording an isolated measurement.
A typical workflow begins by exposing living cells to defined biological, chemical, physical, or material-based conditions. Researchers then quantify changes with imaging, biochemical measurements, or molecular analyses, and compare the results across treatments and controls. The resulting pattern of responses supports evaluation of the tested condition and selection of designs that encourage desired cell behavior.
In bioengineering, screens can test whether a biomaterial is compatible with cells and whether scaffold properties support the desired response. Researchers compare cellular measurements across material or scaffold conditions, using the observed changes to refine designs. This application connects material characteristics with cell behavior during development of biomedical products.
By exposing cells to defined drug or therapeutic conditions, researchers can quantify resulting changes in viability, morphology, proliferation, gene expression, or signaling. Comparing these responses helps assess how cells react to candidate interventions and can inform development of safer, more effective biomedical products. The same framework also supports comparison among alternative treatment conditions.