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In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin

JoVE Journal
Neuroscience
Author Produced

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JoVE Journal Neuroscience

In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin

In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin

DOI:

10.3791/51045-v

•

07:31 min

•

August 24, 2014

•

Mikhail Yuryev, Dmitry Molotkov

¹Neuroscience Center,University of Helsinki

Chapters

00:00Title
01:03Mouse Preparation and Skin Fixation
03:11Imaging
03:47Laser Lesion
04:52Data Analysis and Representative Results
06:30Conclusions

Summary

Automatic Translation

The protocol for dynamic longitudinal imaging and selective laser lesion of nerve endings in reporter transgenic mice is presented.

Tags

Keywords: In Vivo Two-photon Microscopy Nerve Endings Skin Reporter Transgenic Mice EYFP Thy-1 Longitudinal Studies Laser-based Microsurgery

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