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Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
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1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine,The Catholic University of Korea
Chapters
- 00:05Title
- 01:09Isolation of Monocytic Cells from Blood
- 02:40Transduction by Sendai Virus
- 05:46Reprogrammed Cell Maintenance
- 07:20Results: Bright-field Image and Pluripotency of iPSCs Generated from PBMCs
- 08:39Conclusion
We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.
