Oil Immersion Technique
Oil Immersion Technique - Related Videos
11:30
Buoyancy and Drag on Immersed Bodies
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2023
Source: Alexander S Rattner and Sanjay Adhikari; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA Objects, vehicles, and organisms immersed in fluid mediums experience forces from the surrounding fluid in the form of buoyancy- a vertical upward force due to fluid weight, drag- a resistive force opposite the direction of motion, and lift- a force perpendicular to the direction of motion. Prediction and characterization of these forces is...
13:19
Enhanced Oil Recovery using a Combination of Biosurfactants
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Cited by 2 •
2022
We illustrate the methods involved in screening and identification of the biosurfactant producing microbes. Methods for chromatographic characterization and chemical identification of the biosurfactants, determining the industrial applicability of the biosurfactant in enhancing residual oil recovery are also presented.
02:44
Oil Red O Staining: A Technique for Staining Neutral Lipids in Hepatocytes for Detecting Hepatic Steatosis In Vitro
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2025
In this video, we demonstrate in vitro staining of neutral lipids in hepatocytes to detect hepatic steatosis or excessive fat accumulation in liver. Excessive fat is accumulated in the cells in form of lipid droplets. Oil Red O is a lipid soluble dye that binds to intracellular neutral lipids in these lipid droplets. When observed under a light microscope, these intracellular lipid droplets appear intensely red in color.
10:38
Measurement of the Rheology of Crude Oil in Equilibrium with CO2 at Reservoir Conditions
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Cited by 2 •
2017
A method for measuring the rheology of crude oil in equilibrium with carbon dioxide at reservoir conditions is presented.
08:37
Measurement of H2S in Crude Oil and Crude Oil Headspace Using Multidimensional Gas Chromatography, Deans Switching and Sulfur-selective Detection
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Cited by 11 •
2015
A multidimensional gas chromatography method for the analysis of dissolved hydrogen sulfide in liquid crude oil samples is presented. A Deans switch is used to heart-cut light sulfur gases for separation on a secondary column and detection on a sulfur chemiluminescence detector.
FAQs
The critical optical change occurs at the interface between the coverslip and objective. Immersion oil has a refractive index close to that of glass, so light bends less as it leaves the specimen region and enters the lens. This preserves more of the light path for collection, raises the objective’s numerical aperture, and helps separate closely spaced biological features.
Numerical aperture describes the objective’s ability to accept light from the specimen. In oil immersion, reduced refraction at the glass-oil boundary allows more light to enter the high-power objective instead of being diverted away. The resulting increase supports finer resolution, which is especially useful when bacterial structures or intracellular details lie close together.
Without immersion oil, the interface between the coverslip and objective causes more light refraction. The technique instead places a matching medium in that space, improving light collection and resolution rather than merely producing a larger image. This optical difference helps reveal biological detail that may remain unresolved during non-immersion viewing.
Place immersion oil between the specimen’s coverslip and the high-power objective, typically 100×. The oil should occupy the optical path between these surfaces rather than being treated as a general slide coating. After observation, clean both the lens and slide because residual oil can damage equipment and reduce image quality during later work.
The method is most useful when a specimen contains structures too small for ordinary light-microscopy viewing to resolve clearly. It supports examination of bacteria, assessment of cell morphology, and observation of fine intracellular detail. By improving resolution and permitting more accurate observation and measurement, it helps characterize microorganisms and cellular features.
Improved optical resolution can make fine bacterial, cellular, or intracellular features easier to observe and measure. The value lies in resolving detail, not simply increasing apparent image size. Reliable results also depend on maintaining the oil-contact surfaces, so cleaning the objective and slide after use helps preserve image quality and prevent damage.