Oocyte Retrieval
Oocyte Retrieval - Related Videos
08:42
Retrieval of Mouse Oocytes
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Cited by 13 •
2007
This protocol illustrates the technique for extracting oocytes or early-stage fertilized embryos from the oviduct of mice. The ability to identify the infindibulum and insert a blunt end needle into it is essential to correctly performing the procedure.
08:06
Laparoscopic Ovum Pick-up For In Vivo Oocyte Retrieval In Alpacas
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2026
This protocol describes a reproducible method for in vivo oocyte recovery in alpacas using hormonal synchronization, FSH superstimulation, and laparoscopic ovum pick-up (LOPU). The procedure yields consistent follicular responses and efficient oocyte recovery, supporting applications for in vitro embryo production and advanced reproductive biotechnologies.
05:31
OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery
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Cited by 6 •
2021
In vitro maturation (IVM) before gynecological operation (OP-IVM) combines IVM following oocyte retrieval with routine gynecological surgery and serves as an extension of conventional IVM applications for fertility preservation.
13:18
Single Oocyte Bisulfite Mutagenesis
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Cited by 14 •
2012
Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.
12:11
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
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Cited by 21 •
2017
This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.
FAQs
Hormonal stimulation promotes the development of multiple ovarian follicles rather than relying on a single follicle. This increases the number of follicles available for collection and therefore the potential pool of mature eggs. The resulting cohort gives clinicians and embryologists more material to assess for assisted reproduction, fertility preservation, or biological research.
Medication that induces final maturation helps prepare oocytes before collection. Retrieval is therefore timed after this maturation step, so the aspirated follicular fluid can contain oocytes at the intended stage for subsequent laboratory handling. This timing connects ovarian stimulation with the embryology workflow and supports selection of material for fertilization or preservation.
Transvaginal ultrasound guides the retrieval needle toward ovarian follicles during aspiration. This imaging approach allows clinicians to target follicular fluid, which may contain the oocytes, rather than collecting material without visual guidance. The retrieved fluid is then transferred for embryological examination, linking the clinical portion of the procedure to laboratory identification and assessment.
The number and quality of recovered oocytes can influence reproductive outcomes because they determine how much potentially usable material reaches the laboratory. A larger or better-quality collection may provide more oocytes for procedures such as in vitro fertilization, intracytoplasmic sperm injection, or cryopreservation. Embryological assessment is therefore an important step after aspiration.
After aspiration, embryologists examine the follicular fluid to identify the oocytes and assess their condition. The material can then be directed toward in vitro fertilization, intracytoplasmic sperm injection, or cryopreservation, depending on the clinical or preservation goal. This laboratory processing converts the collected sample into material suitable for subsequent reproductive handling or storage.
Oocyte retrieval is used when mature eggs are needed for assisted reproduction or fertility preservation. In assisted reproduction, retrieved oocytes may undergo in vitro fertilization or intracytoplasmic sperm injection. For preservation, they can be cryopreserved for later use. The same collection process therefore supports different reproductive strategies while relying on subsequent embryological assessment.
The procedure provides access to human oocytes and associated follicular material for studying oocyte biology. Researchers can examine retrieved cells after embryologists identify and assess them, while the clinical workflow supplies material generated through controlled ovarian stimulation and timed maturation. This connection helps relate observations of oocyte biology to fertility treatment and preservation strategies.