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JoVE Journal
Developmental Biology

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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
 

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Article doi: 10.3791/55242
May 11th, 2017

Summary May 11th, 2017

This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.

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