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Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
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Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

DOI:
10.3791/62005-v

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09:00 min

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January 25, 2021

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, ,
1Department of Molecular Medicine,The Scripps Research Institute, La Jolla, CA, USA, 2Department of Medicine,University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

Chapters

  • 00:04Introduction
  • 01:32Adipose Depot Collection and Digestion
  • 03:28Plating Preadipocytes
  • 04:56Preadipocyte Culture Expansion
  • 06:17White and Brown Preadipocyte Differentiation
  • 06:55Results: Representative Adipocyte Progenitor Collection and Differentiation
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

This report describes a protocol for the simultaneous isolation of primary brown and white preadipocytes from newborn mice. Isolated cells can be grown in culture and induced to differentiate into fully mature white and brown adipocytes. The method enables genetic, molecular, and functional characterization of primary fat cells in culture.

Tags

Keywords: PreadipocytesAdipocyte IsolationAdipocyte DifferentiationNewborn MiceObesityDiabetesCell Culture Model

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