Zebrafish Sensory Epithelia
Zebrafish Sensory Epithelia - Related Videos
08:03
Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio)
0 Views •
Cited by 25 •
2009
The inner ear sensory epithelium of adult zebrafish is a good model system for understanding the mechanisms of hair cell regeneration in adult vertebrates. This protocol demonstrates the fine dissection of the epithelia, through which we can get tissue samples for studying the regenerative events at cellular and subcellular levels.
03:55
Differentiating Otic Progenitor Cells Into Sensory Epithelial Cells Followed by Immunostaining
0 Views •
2025
This video demonstrates the differentiation of immortalized multipotent otic progenitor (iMOP) cells into sensory epithelial cells. It outlines the steps involved in culturing iMOP cells, forming otospheres, inducing differentiation, and visualizing cellular components and differentiation markers through fluorescence microscopy to confirm successful cell differentiation.
07:27
Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration
0 Views •
Cited by 43 •
2011
Acute kidney injury (AKI) in humans is a common clinical problem caused by damage to the epithelial cells that comprise kidney nephrons, and AKI is associated with high mortality rates of 50-70%1. Following epithelial cell destruction, nephrons have a limited ability to regenerate, though the mechanisms and limitations that guide this phenomenon remain poorly understood. In this video article, we describe our technique for targeted laser ablation of kidney nephron cells in the zebrafish...
01:31
What is a Sensory System?
0 Views •
2019
Sensory systems detect stimuli—such as light and sound waves—and transduce them into neural signals that can be interpreted by the nervous system. In addition to external stimuli detected by the senses, some sensory systems detect internal stimuli—such as the proprioceptors in muscles and tendons that send feedback about limb position. Sensory systems include the visual, auditory, gustatory (taste), olfactory (smell), somatosensory (touch, pain, temperature, and proprioception), and vestibular...
13:11
Sensory Exam
0 Views •
2023
Source:Tracey A. Milligan, MD; Tamara B. Kaplan, MD; Neurology, Brigham and Women's/Massachusetts General Hospital, Boston, Massachusetts, USA A complete sensory examination consists of testing primary sensory modalities as well as cortical sensory function. Primary sensory modalities include pain, temperature, light touch, vibration, and joint position sense. Sensation of the face is discussed in the videos Cranial Nerves Exam I and II, as are the special senses of smell, vision, taste, and...
FAQs
At the apical surface of a hair cell, stimulus-driven force bends the hair bundle. That mechanical displacement opens mechanically gated ion channels, allowing the cell to convert movement into an electrical signal. In this way, the direction and magnitude of bundle deflection link environmental motion with receptor-cell activity, providing a cellular basis for sensory transduction in the inner ear and lateral-line system.
The two systems use comparable hair-cell transduction machinery but receive different physical inputs. Inner-ear epithelia respond to fluid movement associated with hearing and balance, whereas lateral-line epithelia detect water displacement around the fish. This distinction allows zebrafish to connect a shared cellular mechanism with different sensory roles and makes both systems useful for studying mechanosensation.
Supporting cells are important because they can contribute to replacing hair cells after injury. Their regenerative capacity provides a way to investigate how sensory epithelia respond to damage and restore receptor-cell populations. Comparisons with mammals are especially informative because this replacement ability is limited in mammals, helping researchers examine cellular mechanisms that may underlie differences in sensory repair.
Studies of these epithelia can address several linked processes, including sensory transduction, hair-cell regeneration, developmental patterning, and cellular responses to injury. Examining these processes in the inner ear and lateral line connects molecular or cellular events with tissue-level sensory function. The resulting knowledge helps clarify how hearing and balance disorders relate to disrupted receptor cells or repair responses.
Researchers can use the injury-response capacity of zebrafish sensory epithelia to examine what happens after hair-cell damage and how supporting cells participate in replacement. This context makes the tissues useful for studying repair within an intact sensory system rather than considering regeneration only as an isolated cellular event. The findings provide biological context for understanding why comparable repair is restricted in mammals.
Hearing and balance depend on mechanosensory hair cells that translate movement into electrical signals, so damage to these cells or disruption of their supporting tissue can affect sensory function. Zebrafish offer a model in which transduction, development, injury responses, and hair-cell replacement can be considered together. This broad view supports investigation of cellular processes associated with sensory disorders.