Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio)

In the inner ear of zebrafish, there are three pairs of sac, like end organs, the utricle, the S, and the Lana. The utricle locates most anteriorly while the S and the lega closely attached to each other and locate posteriorly to the utricle After gross dissection, the S and lega stay attached and the stonelike structure otolith can be easily seen in each end organ in the following fine dissection. The S and lega are separated from each other by cutting from the ventral side.

Otoliths are removed and the nons sensory epithelial tissues are trimmed off to get sensory epithelial tissues from all three end organs. Hi, I'm Jean Leon from the Lab of Sean Burgess in the developmental genomics section of the National Human Genome Research Institute. Today we'll show you a procedure for the fine and grass dissection of the inner year sensory epithelia from the adult zebra zebrafish.