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Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
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Neuroscience
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JoVE Journal Neuroscience
Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction

Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction

DOI:
10.3791/53207-v

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08:41 min

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January 12, 2016

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, ,
1Centre de Recherche,Hospital du Sacre-Coeur de Montreal

Chapters

  • 00:05Title
  • 00:52Myocardial Infarction Surgery
  • 03:36Isolation of the Amygdala
  • 05:23Caspase-3 Activity Assay
  • 07:39Results: Caspase-3 Activity in the Amygdala of Rats After Myocardial Infarction
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

Myocardial infarction (MI) is not only followed by impaired cardiac function but also by apoptosis in the amygdala, a brain region involved in the behavioral consequences of MI. This protocol describes how to induce MI, collect amygdala tissue and measure caspase-3 activity, a marker of apoptosis, therein.

Tags

Keywords: Caspase-3 ActivityRat AmygdalaSpectrofluorometryMyocardial InfarctionBehavioral Neural ScienceCognitive PerformanceMental HealthAgingSleepSurgeryAnesthesiaIntubationSurgical ProcedureCoronary OcclusionSutureHemostatic ClampThoracic CavityPneumothorax

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