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JoVE Journal
Developmental Biology

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Culture and Transfection of Zebrafish Primary Cells

Culture and Transfection of Zebrafish Primary Cells

Article DOI: 10.3791/57872 08:51 min August 17th, 2018
August 17th, 2018



We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.



Zebrafish Primary Cells Culture Transfection Intercellular Dynamics In Vivo Analysis Zebrafish Cell Lines Protocol Zebrafish Embryos Fertilization Petri Dish Danieau 30% Methylene Blue Incubate Chorions Pronase Silicone Grease
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