Culture and Transfection of Zebrafish Primary Cells

This method provides the zebrafish community a tool for the the analysis of intercellular dynamics with a resolution unattainable in vivo. And also it allows to compensate for the lack of zebrafish cell lines. The main advantage of this technique is that you isolated and tranfect those primary cells directly from the original tissue so you do not need to keep cell lines.

To begin the protocol prepare the zebrafish embryos two days post fertilization, or dpf. On day one set up several crossings of the zebrafish strain of choice. On day two mate to the fish and collect the eggs directly after spawning in a plastic 10 centimeter Petri dish.