Zebrafish Influenza Model
Zebrafish Influenza Model - Related Videos
09:07
Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses
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Cited by 16 •
2017
Systemic and localized zebrafish infection models for human influenza A virus are demonstrated. Using a systemic infection model, zebrafish can be used to screen antiviral drugs. Using a localized infection model, zebrafish can be used to characterize host immune cell responses.
10:44
Influenza A Virus Studies in a Mouse Model of Infection
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Cited by 38 •
2017
Influenza A viruses (IAVs) are important human respiratory pathogens. To understand the pathogenicity of IAVs and to perform preclinical testing of novel vaccine approaches, animal models mimicking human physiology are required. Here, we describe techniques to evaluate IAV pathogenesis, humoral responses and vaccine efficacy using a mouse model of infection.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
07:45
Modeling Dysplastic and Functional Lung Alveolar Repair after Influenza Infection
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2025
This protocol provides instructions for intranasal administration of IAV and downstream analysis of mouse lung damage and associated transcriptional changes to lung cell types.
09:31
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
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Cited by 56 •
2017
We describe the use of a phenotypic fluorescence-based neuraminidase inhibition assay to assess the susceptibility of influenza A and B viruses to the neuraminidase inhibitor class of antivirals.
FAQs
Live imaging allows researchers to observe immune-cell recruitment and behavior as infection-related responses develop in transparent zebrafish embryos and larvae. Instead of relying only on endpoint measurements, investigators can connect cellular movement and inflammatory activity with tissue responses over time, helping relate microscopic immune events to outcomes across the organism.
Genetic analysis helps investigators examine how particular host responses contribute to influenza-related inflammation and tissue effects. Used alongside imaging, it can connect changes in immune mechanisms with visible cellular behavior and whole-organism responses. This combination supports a more integrated interpretation of host-pathogen interactions than either observation or genetic assessment alone.
These readouts show how the host responds after influenza virus or an infection-related stimulus is introduced. Changes in inflammatory signaling, immune-cell recruitment, and cell behavior provide complementary information about the developing response, while tissue observations indicate how that response affects the organism. Together, they help clarify the relationship between immunity and infection-associated damage.
A study generally begins with zebrafish embryos or larvae, followed by introduction of influenza virus or an infection-related stimulus. Researchers then monitor immune-cell behavior, inflammatory signaling, and tissue responses using live imaging and genetic analysis. The resulting observations can be compared across experimental conditions to assess host responses and intervention-related effects.
Their external development and optical accessibility make embryos and larvae practical for observing infection-related cellular events directly. These features also support rapid experimental screening, allowing researchers to examine host responses and potential interventions in a whole-organism setting. The model therefore links visible immune activity with broader tissue and organismal outcomes.
Researchers can introduce influenza virus or related stimuli, apply a potential intervention, and then assess changes in immune-cell recruitment, inflammatory signaling, and tissue responses. Comparing these readouts across conditions indicates whether an intervention alters host-pathogen interactions or inflammation. The model is especially useful when rapid screening and organism-level observation are important.