Seahorse Xfe
Seahorse Xfe - Related Videos
10:39
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
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Cited by 51 •
2015
The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.
11:27
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Cited by 24 •
2015
Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.
07:20
Bioenergetic Profile Experiment using C2C12 Myoblast Cells
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Cited by 190 •
2010
A description of a method for profiling mitochondrial function in cells is provided. The mitochondrial profile generated provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity.
07:45
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
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Cited by 275 •
2015
Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
FAQs
Oxygen consumption rate provides an indicator of mitochondrial respiration, whereas extracellular acidification rate indicates glycolytic activity. Examining both measurements helps researchers compare how cells produce and use energy through distinct metabolic processes. This paired view can reveal whether a cellular state is associated more strongly with respiratory activity, glycolytic activity, or changes in both pathways.
Controlled additions create defined metabolic challenges and allow cellular responses to be measured in real time. Researchers can compare measurements before and after each addition to determine how a substrate, inhibitor, or compound changes respiration or glycolytic activity. These response patterns help connect a specific intervention with altered cellular energy metabolism rather than relying on a single endpoint measurement.
Considering oxygen consumption and extracellular acidification together provides a broader picture of cellular energy metabolism than either measurement alone. A change in one indicator may suggest altered mitochondrial respiration, while a change in the other may point to modified glycolytic activity. Linking these metabolic patterns with cellular behavior supports interpretation of health- and disease-associated cellular states.
A typical workflow places cells in a microplate-based assay, records metabolic measurements, and then introduces selected substrates, inhibitors, or other compounds under controlled conditions. Measurements collected before and after each addition provide a real-time comparison of cellular responses. The resulting changes in oxygen consumption rate and extracellular acidification rate are then interpreted as indicators of metabolic activity.
Medical researchers can apply Seahorse XFe assays to investigate cancer metabolism, immune-cell function, and mitochondrial disorders. The measurements help characterize how disease-associated cells produce and use energy, while controlled compound additions can probe their metabolic responses. This information supports disease characterization and may help distinguish metabolic features relevant to diagnosis-oriented research or therapeutic development.
By measuring metabolic changes before and after exposure to a compound, researchers can assess whether an intervention alters mitochondrial respiration, glycolytic activity, or both. Such results provide evidence about drug mechanisms and cellular treatment responses. In medicine, these findings can contribute to evaluating potential therapeutic strategies and to understanding how metabolic changes relate to cellular behavior.