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Immunology and Infection
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Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

DOI:
10.3791/53424-v

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07:45 min

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November 28, 2015

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, ,
1Department of Medical Biochemistry, Experimental Vascular Biology,Academic Medical Center, Amsterdam

Chapters

  • 00:05Title
  • 00:59Bone Marrow-derived Macrophage Polarization
  • 02:17Extracellular Flux Assay Preparation
  • 03:46Bioenergetic Characterization of Polarized M1 and M2 Macrophages
  • 05:17Results: Metabolic Characteristics of Control M0 and M1- and M2-polarized Macrophages
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.

Tags

Keywords: Macrophage PolarizationM1 MacrophagesM2 MacrophagesMetabolic ReprogrammingGlycolysisOxidative PhosphorylationExtracellular Flux AnalysisOxygen Consumption RateExtracellular Acidification RateMitochondrial ActivitySpare Respiratory Capacity

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