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Chapters
- 00:00Title
- 01:15Introduction
- 03:01Plasmid DNA Constructions
- 04:04Streak Analysis of the Slow Growth Phenotype Restoration in WRN Transformed sgs1 top3 Strain
- 06:00Genetic Analysis of Hydroxyurea (HU) or Methylmethane-sulfonate (MMS) Sensitivity in WRN Transformed Yeast Strains
- 07:24Determining Cell Cycle Distribution of the WRN Transformated sgs1 top3 Strain
- 09:21Preparing Cell Lysates for Western Blot Analysis
- 11:09Effects of WRN Expression in sgs1 and top3 Yeast Cells
- 13:23Conclusion
Genetic studies in yeast can be employed to investigate the molecular and cellular functions of human genes in cellular DNA metabolism. Methods are described for the genetic characterization of the human WRN gene product defective in the premature aging disorder Werner syndrome in functionally conserved pathways using yeast as a tractable model system.
Tags
Genetic StudiesHuman DNA Repair ProteinsYeast Model SystemMammalian SystemsModel Genetic SystemsLower EukaryotesWerner Syndrome Helicase-nuclease (WRN)Nucleic Acid MetabolismCellular PhenotypesGenetic Mutant BackgroundsCatalytic ActivitiesProtein InteractionsWRN GeneDNA PlasmidsRegulatory Plasmid ElementYeast Mutant BackgroundGenomic Stability
