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Single-cell Photoconversion in Living Intact Zebrafish
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Developmental Biology
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JoVE Journal Developmental Biology
Single-cell Photoconversion in Living Intact Zebrafish

Single-cell Photoconversion in Living Intact Zebrafish

DOI:
10.3791/57024-v

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09:49 min

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March 19, 2018

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,
1Department of Biological Sciences,University of Notre Dame, 2Center for Stem Cells and Regenerative Medicine,University of Notre Dame

Chapters

  • 00:05Title
  • 00:39Preparation of Zebrafish Specimen
  • 02:12Microscope Mounting and Pre-conversion Imaging
  • 03:35Single-cell Photoconversion
  • 06:18Post-photoconversion Imaging
  • 07:38Results: Photoconverted Eos Protein in Single and a Population of Ganglion Cells in Transgenic Zebrafish
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to show how cell photoconversion is achieved through UV exposure to specific areas expressing the fluorescent protein, Eos, in living animals.

Tags

Keywords: Single-cell PhotoconversionZebrafishFluorescent Cell PopulationsDevelopmental QuestionsSingle-cell MigrationMinimally InvasivePhotoconvertible Protein488 Nanometer Light SourceGFP Filter SetsDechorionateLow Melting Point AgaroseAnesthetized Transgenic Sox10 Eos Positive FishConfocal MicroscopyPre-conversion ImagingDorsal Root GanglionC488 Laser3D Capture

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