Single-cell Photoconversion in Living Intact Zebrafish

The overall goal of this photoconversion technique is to distinguish single cells in a single animal from other fluorescent cell populations. This method can help answer key developmental questions in the field, and allow us to visualize single-cell migration over time. The main advantage of this technique is that it is minimally invasive and can be used to label single cells or larger populations.

Additionally, the user can select the specific region of interest and photo convert the desired target. After mating male and female transgenic zebrafish expressing a photoconvertible protein and collecting the eggs according to the text protocol, use a dissecting microscope with a 488 nanometer light source and GFP filter sets to screen 24 hpf embryos. Using a needle or tweezers, manually dechorionate the embryos.