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High-Throughput Quantification of the Synthesis and Distribution of mtDNA

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Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution (Video) | JoVE

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Biochemistry

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JoVE Journal Biochemistry

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution (Video) | JoVE

High-Throughput Quantification of the Synthesis and Distribution of mtDNA

DOI:

10.3791/200043-v

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02:04 min

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May 05, 2023

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Lukasz S. Borowski², Karolina Kasztelan³, Jolanta Czerwinska-Kostrzewska², Roman J. Szczesny

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

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Keywords: Primary Antibody Secondary Antibody Transfected HeLa Cells Mitochondrial DNA Distribution BSA PBS Alexa Fluor 488 Alexa Fluor 555 Anti-DNA Antibodies Mitochondrial Transcription Factor A (TFAM)Fluorescence Quantification

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