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Journal
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Genetics
/
A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
/
DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE
JoVE Journal
Genetics
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JoVE Journal
Genetics
dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE
A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
DOI:
10.3791/200139-v
•
03:34 min
•
April 21, 2023
•
Llorenç Rovirosa*
1
,
Laureano Tomás-Daza*
1,2
,
Blanca Urmeneta
,
Alfonso Valencia
3
,
Biola M. Javierre
4
1
Josep Carreras Leukaemia Research Institute
,
2
Barcelona Supercomputing Center
,
3
Catalan Institution for Research and Advanced Studies (ICREA)
,
4
Institute for Health Science Research Germans Trias i Pujol
Tags
Keywords: DATP-Tailing
Adapter Ligation
PCR Amplification
Scarce Cell DNA
Biotin
Klenow Exo-minus
Ligation Buffer
T4 DNA Ligase
Paramagnetic Bead Purification
Double-Sided Selection
TLE Buffer
70% Ethanol
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