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Journal
/
Biology
/
Stria Vascularis Microdissection for Single-Nucleus Sequencing or Immunostaining
/
Preparation of Stria Vascularis Single-Nucleus Suspension (Video) | JoVE
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of Stria Vascularis Single-Nucleus Suspension
Preparation of Stria Vascularis Single-Nucleus Suspension (Video) | JoVE
Stria Vascularis Microdissection for Single-Nucleus Sequencing or Immunostaining
DOI:
10.3791/200195-v
•
01:28 min
•
April 21, 2023
•
Dillon Strepay
,
Rafal Olszewski
,
Ian Taukulis
,
J. Dixon Johns
,
Shoujun Gu
,
Michael Hoa
1
Auditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders
,
National Institutes of Health
Tags
Keywords: Stria Vascularis
Single-nucleus Suspension
Homogenize
Dounce Homogenizer
Lyse
Filter
Centrifuge
Supernatant
Resuspend
Nuclei Wash
Resuspension Buffer
Trypan Blue
Single Nuclei Captures
Cell Density
Cell Suspension
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