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Journal
/
Biology
/
Production and Imaging of Recombinant Protein-Filled Vesicles from
E. coli
/
Rekombinant vesikkelisolasjon fra bakteriekultur for å frigjøre løselig protein
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein
Rekombinant vesikkelisolasjon fra bakteriekultur for å frigjøre løselig protein
Production and Imaging of Recombinant Protein-Filled Vesicles from
E. coli
DOI:
10.3791/200248-v
•
02:50 min
•
June 30, 2023
•
Bree R. Streather
,
Karen Baker
,
Tara A. Eastwood
,
Daniel P. Mulvihill
1
School of Biosciences
,
University of Kent
Tags
Keywords: Recombinant Vesicle
Bacterial Culture
Soluble Protein
VNP Sequence Tag
Lysogeny Broth
Terrific Broth
IPTG
Centrifugation
Filtration
Vesicle Concentration
Sonication
BL21DE3 Escherichia Coli
MNeongreen
SDS-PAGE
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