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Journal
/
Biology
/
Production and Imaging of Recombinant Protein-Filled Vesicles from
E. coli
/
Çözünür Proteini Serbest Bırakmak için Bakteriyel Kültürden Rekombinant Vezikül İzolasyonu
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein
Please note that all translations are automatically generated.
Click here for the English version.
Çözünür Proteini Serbest Bırakmak için Bakteriyel Kültürden Rekombinant Vezikül İzolasyonu
Production and Imaging of Recombinant Protein-Filled Vesicles from
E. coli
DOI:
10.3791/200248-v
•
02:50 min
•
June 30, 2023
•
Bree R. Streather
,
Karen Baker
,
Tara A. Eastwood
,
Daniel P. Mulvihill
1
School of Biosciences
,
University of Kent
Tags
Keywords: Recombinant Vesicle
Bacterial Culture
Soluble Protein
VNP Sequence Tag
Lysogeny Broth
Terrific Broth
IPTG
Centrifugation
Filtration
Vesicle Concentration
Sonication
BL21DE3 Escherichia Coli
MNeongreen
SDS-PAGE
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