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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

単球単離と単球由来樹状細胞への単球分化

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Journal (Immunology and Infection)

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Journal (Immunology and Infection)

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

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単球単離と単球由来樹状細胞への単球分化

De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

Article DOI: 10.3791/200350-v • 06:26 min • October 20th, 2023

October 20th, 2023 •

Vanessa C. C. Luz^1,2, Zélia Silva^1,2, Patrícia Sobral^1,2,3, Ankit Tanwar^4,5, Rachel L. Paterson⁶, Paula A. Videira^1,2,7

¹Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, ²UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, ³LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, ⁴Department of Microbiology and Immunology, Albert Einstein College of Medicine, ⁵Department of Oncology, Albert Einstein College of Medicine, ⁶Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, ⁷CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

Tags

単球由来樹状細胞、シアル化表現型、シアル酸、免疫応答調節、免疫サーベイランス、糖鎖免疫チェックポイント、免疫療法、樹状細胞(DC)、抗原提示細胞(APC)、DCの成熟、シアル酸含量、T細胞活性化

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