De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

October 20, 2023

Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface (Video) | JoVE

03:17 min

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Monocyte Isolation and Monocyte Differentiation into Monocyte-Derived Dendritic Cells

06:26 min

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Treatment of the Monocyte-Derived Dendritic Cells (Mo-DCs) with Sialidase (Video) | JoVE

03:03 min

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Maturation Profiling of Human Monocyte-Derived Dendritic Cells (Mo-DCs) (Video) | JoVE

01:29 min

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Vanessa C. C. Luz², Zélia Silva², Patrícia Sobral^2,3, Ankit Tanwar⁵, Rachel L. Paterson, Paula A. Videira^2,7

¹Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ²UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ³LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,Universidade NOVA de Lisboa, ⁴Department of Microbiology and Immunology,Albert Einstein College of Medicine, ⁵Department of Oncology,Albert Einstein College of Medicine, ⁶Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark,Zona Industrial da Gandra, ⁷CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa

Summary

A unique, comprehensive protocol to generate de-sialylated human monocyte-derived dendritic cells (mo-DCs) from isolated peripheral blood mononuclear cells (PBMCs) using a sialidase treatment is presented. Further, methods to assess the phenotypic and functional characterization of mo-DCs and evaluate how sialidase treatment improves the maturation level of mo-DCs are described.