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Journal
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Biology
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Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
/
이미징을 위한 효모 세포 준비, 이미징 조건 최적화 및 이미지 수집
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection
Please note that all translations are automatically generated.
Click here for the English version.
이미징을 위한 효모 세포 준비, 이미징 조건 최적화 및 이미지 수집
Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
DOI:
10.3791/200471-v
•
02:33 min
•
June 02, 2023
•
Emily Jie-Ning Yang
,
Istvan R. Boldogh
2
,
Haojie Ji
,
Liza Pon
2
,
Theresa C. Swayne
2
1
Department of Pathology and Cell Biology
,
Columbia University Irving Medical Center
,
2
Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center
,
Columbia University Irving Medical Center
Tags
Keywords: Yeast Cells
Mitochondria
HyPer7
Microscopy
Imaging
Fluorescence
Confocal Microscopy
SCMOS Camera
Binning
Laser Power
Exposure
Dynamic Range
Noise Level
Time-lapse
Z-stacks
Transmitted Light
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check_url/200471?article_type=v
살아있는 효모 세포에서 미토콘드리아 과산화물을 위한 비율계량 바이오센서인 mtHyPer7의 이미징
check_url/200471?article_type=v
이미징을 위한 효모 세포 준비, 이미징 조건 최적화 및 이미지 수집
check_url/200471?article_type=v
스크립트를 사용한 효모 세포의 mtHyPer7 반자동 분석
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