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Journal
/
Cancer Research
/
Genome-Scale Screening to Identify and Compare Therapeutic Targets in Tumors
/
Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens (Video) | JoVE
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens
Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens (Video) | JoVE
Genome-Scale Screening to Identify and Compare Therapeutic Targets in Tumors
DOI:
10.3791/200477-v
•
03:15 min
•
August 25, 2023
•
Brittany Turner-Ivey
,
Jody Fromm Longo
,
Dorea P. Jenkins
,
Stephen T. Guest
,
Steven L. Carroll
2
1
Hollings Cancer Center
,
Medical University of South Carolina
,
2
Department of Pathology and Laboratory Medicine
,
Medical University of South Carolina
,
3
Department of Computational Medicine and Bioinformatics
,
University of Michigan, Ann Arbor
Tags
Keywords: Lentiviral Packaging
Viral Titering
Genome-scale ShRNA Screens
293T Cells
Packaging Plasmid Mix
Plasmid Barcode Library
Transfection Reagent
Viral Particles
Viral Titering
Cationic Polymer
Tumor Cell Growth Media
Tumor Cells
Lentivirus
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