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Journal
/
Biology
/
Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells
/
Mesenchymal Stem Cell Surface Staining for Immunophenotyping (Video) | JoVE
JoVE Journal
Biology
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JoVE Journal
Biology
Mesenchymal Stem Cell Surface Staining for Immunophenotyping
Mesenchymal Stem Cell Surface Staining for Immunophenotyping (Video) | JoVE
Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells
DOI:
10.3791/200703-v
•
02:33 min
•
November 10, 2023
•
Ayona Gupta*
1
,
Risani Mukhopadhyay*
1
,
Himanshi Khandelwal
,
Narendra Nala
,
Uttara Chakraborty
1
Manipal Institute of Regenerative Medicine, Bengaluru
,
Manipal Academy of Higher Education, Manipal
,
2
HIV-AIDS Laboratory, Molecular Biology and Genetics Unit
,
Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)
Tags
Keywords: Mesenchymal Stem Cells
Cell Surface Staining
Immunophenotyping
Trypsinization
Cell Pellet
Cell Suspension
Staining Buffer
Compensation Controls
Single Stain Tubes
Centrifugation
DAPI
Antibody Staining
Cell Preparation
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