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Journal
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Immunology and Infection
/
Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses
/
Ensayo de producción de virus de pseudotipo SARS-CoV-2 y neutralización sérica
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay
Please note that all translations are automatically generated.
Click here for the English version.
Ensayo de producción de virus de pseudotipo SARS-CoV-2 y neutralización sérica
Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses
DOI:
10.3791/200835-v
•
04:50 min
•
November 21, 2023
•
Tobia Fantoni*
1
,
Michele Bissoli*
1
,
Chiara Stefani
,
Mauro Voi
,
Alexandrina Dabija
,
Rebecca Casula
,
Domenico Luca Minafra
,
Julys da Fonseca Palmeira
,
Enrique Roberto Argañaraz
,
Martin Mayora-Neto
,
Nigel J. Temperton
,
Donato Zipeto
,
Alessandra Ruggiero
1
Department of Neuroscience, Biomedicine and Movement
,
University of Verona
,
2
Laboratory of Molecular Neurovirology, Faculty of Health Science
,
University of Brasília
,
3
Viral Pseudotype Unit, Medway School of Pharmacy
,
Universities of Kent and Greenwich at Medway
Tags
Keywords: SARS-CoV-2
Pseudotype Virus
Serum Neutralization Assay
HEK 293T Cells
Transfection
Polyethylenimine
Cell Culture
Virus Production
Viral Supernatant
Serial Dilution
HEK 293T ACE2 Cells
Luciferase Assay
Serum Samples
Heat Inactivation
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