Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

November 21, 2023

Studying Host-Virus Interactions with Pseudotyped Viruses (Video) | JoVE

01:03 min

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SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay (Video) | JoVE

04:50 min

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Tobia Fantoni*¹, Michele Bissoli*¹, Chiara Stefani, Mauro Voi, Alexandrina Dabija, Rebecca Casula, Domenico Luca Minafra, Julys da Fonseca Palmeira, Enrique Roberto Argañaraz, Martin Mayora-Neto, Nigel J. Temperton, Donato Zipeto, Alessandra Ruggiero

¹Department of Neuroscience, Biomedicine and Movement,University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science,University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy,Universities of Kent and Greenwich at Medway

Summary

Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients’ serum after COVID-19 vaccination.

Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

Studying Host-Virus Interactions with Pseudotyped Viruses (Video) | JoVE

SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay (Video) | JoVE

Summary

Article