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Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method
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Chapters
- 00:00Title
- 01:03Introduction
- 01:34Establishing a Glial Cell Culture
- 04:29Isolation, Purification, and Cuturing of Dorsal Root Ganglion Neurons (DRGs) and Coculturing of DRGs with Glial Cells
- 07:29Representative Cultures of Glial Cells, DRG Neurons, and Cocultures of Both Cell Types
- 08:36Conclusion
In this protocol, we described a new method to study the influence of glial cell heterogeneity on axon growth with an in vitro co-culture system. Rat cortical glial cells were cultured to confluence and cocultured with highly purified rat dorsal root ganglia neurons. Different glial cell influence on neurons adhesion and axon growth was compared directly in the same culture. This method provides a new way to directly study the glial cell heterogeneity influence on neuron adhesion and axon growth.
