JoVE Encyclopedia of Experiments
Neuroscience
0 views • 1:31 min • August 7th, 2025
Take adhered murine microglial cells in a multiwell plate.
Add polyacrylic acid-coated magnetic nanoparticles (MNPs) to the test well, leaving another well untreated as a control.
Incubate the cells to allow MNP uptake via endocytosis, enabling their internalization.
Wash with buffer to remove unbound MNPs.
Add an enzyme solution to detach the cells.
Transfer the detached cells to tubes and centrifuge to pellet them.
Discard the supernatant, resuspend the pellet in basal media, and transfer the suspension to microcentrifuge tubes.
Place the tubes in the center of a copper coil within the magnetic applicator device.
The coil generates an alternating magnetic field (AMF) while the applicator maintains a constant target temperature.
The AMF interacts with the MNPs to generate heat, disrupting cellular structures and triggering cell death pathways. In contrast, control cells without MNPs show no thermal damage.
A decrease in the viability of MNP-treated cells confirms thermal damage due to magnetic hyperthermia.