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The Successive Alleys Test of Anxiety in Mice and Rats

The Successive Alleys Test of Anxiety in Mice and Rats

Article DOI: 10.3791/2705-v 05:05 min June 17th, 2013
June 17th, 2013



The plus-maze measures anxiety-like behaviour in rodents. There are two opposite closed and two opposite open arms; anxious rodents avoid the open arms. The central area is neither completely open nor closed, so time spent here is ambiguous and difficult to interpret. Here a modification of the plus-maze protocol eliminating this area is described.


Successive Alleys Test Anxiety Mice Rats Plus-maze Montgomery Handley Mithani File Lister Open Arms Enclosed Arms Anxiety Index Central Area Decision Making Shepherd Et Al. Zero Maze Modification Light-dark Box Emergence Tests Anxiogenic Properties
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