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Journal / Biology / Kalite İnositol Pyrophosphates hazırlanması
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Kalite İnositol Pyrophosphates hazırlanması
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Kalite İnositol Pyrophosphates hazırlanması

Article DOI: 10.3791/3027-v • 10:35 min • September 3rd, 2011
September 3rd, 2011 •
1, 1, 1, 1, 1
1Medical Research Council (MRC), Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London

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İnositol pyrophosphates kanser, diyabet ve obezitenin insan patolojilerin önemli bir rol oynar; ancak, etki mekanizması tam bir anlaşmazlık meselesidir. Piyasada bulunan inositol pyrophosphates eksikliği detaylı çalışmalar sorunlu vermektedir. Burada inositol pyrophosphates miligram üretmek ve izole etmek için basit bir protokol.

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Moleküler Biyoloji Sayı 55 Polyacrilamyde Jel Elektroforezi (PAGE) inositol hexakisphosphate (IP6) fitik asit diphosphoinositol pentakisphosphate (IP7) bisdiphoshoinositol tetrakisphosphate (IP8) IP6-kinaz (IP6K) PP-IP5K VIP1
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