JoVE Encyclopedia of Experiments
Neuroscience
0 views • 4:19 min • July 8th, 2025
Begin with an electrophysiological setup with a sagittal brain section submerged in aCSF containing synaptic blockers.
This section includes a neuron-rich PPN region.
The synaptic blockers inhibit various neuronal excitatory and inhibitory receptors and membrane sodium channels, preventing their interference.
Fill a patch pipette with a potassium-rich solution and insert it into a holder connected to a recording electrode and an amplifier.
Apply positive air pressure to the pipette to prevent clogging, then position it near a PPN neuron.
Apply a negative suction to form a tight seal with the neuron membrane and continue this suction until the membrane breaks.
Deliver the positive current pulses to open the voltage-dependent calcium channels, allowing calcium ions to enter.
This induces gamma-band oscillations, a low-level excitation that is essential for wakefulness without fully exciting the neurons.
Record these signals via the recording pipette to monitor gamma-band oscillation.
In this procedure, transfer a slice to the submersion chamber, place a screen on top of it to hold the slice down, and perfuse it at 1.5 milliliters per minute, with oxygenated aCSF containing the selec
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