Assessing the Effects of Toxins on Chick Embryo Neural Network Development Using Multielectrode Arrays

Begin with adherent cultures of early embryonic chick neurons on extracellular matrix-coated multielectrode arrays, or MEAs.

The base of the MEAs contains grids of tiny electrodes that record neuronal activity.

In the test culture, neurons grow in the presence of a toxin, while in the control culture, neurons grow without the toxin.

Before recording, replace the media in both MEAs with toxin-free media and incubate briefly.

Next, place one MEA in the recording device and begin recording.

Neurons communicate via electrical impulses called action potentials or spikes.

At the synapse, these action potentials trigger neurotransmitter release, propagating the signal to the next neuron.

The MEA electrodes record the spikes generated by the synaptically connected neurons. 

In the control condition, the healthy mature neuronal network exhibits synchronous spiking activity.

In the test culture, reduced spiking and synchrony suggest impaired neuronal network maturation caused by the toxin.

On the day of acquisition, replace the culture medium with fresh neurobasal medium, and return the arrays to the CO₂ incubator for a minimum of two hours before recording. Start the recording software and