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Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

DOI:
10.3791/52361-v

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15:05 min

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February 05, 2015

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, , ,
1Research Division, Cellular Molecular Biology Branch,United States Army Medical Research Institute of Chemical Defense

Chapters

  • 00:05Title
  • 01:49Passaging and Maintenance of Embryonic Stem Cells (ESCs)
  • 03:42Neuronal Differentiation
  • 08:26Measured Inhibition of Synaptic Transmission (MIST) Assay for Quantifying Miniature Excitatory Post-synaptic Currents
  • 12:02Results: Synaptic Activity in ESNs Measured by the MIST Assay
  • 14:13Conclusion

Summary

Automatic Translation

Automatic Translation

A custom protocol is described to differentiate mouse ES cells into defined populations of highly pure neurons exhibiting functioning synapses and emergent network behavior. Electrophysiological analysis demonstrates the loss of synaptic transmission following exposure to botulinum neurotoxin serotypes /A-/G and tetanus neurotoxin.

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Keywords: Stem Cell-derived NeuronsCentral Nervous SystemNeurotoxinsClostridial NeurotoxinsBotulinum NeurotoxinTetanus NeurotoxinSynaptic ActivityElectrophysiologyMiniature Excitatory Post-synaptic CurrentsDose-dependent EffectsIn Vitro Model

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