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Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI
 

Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI

Article DOI: 10.3791/3362
January 30th, 2012

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Summary January 30th, 2012

We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy.

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