JoVE Journal
Biology
Biology
A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Chapters
Summary January 30th, 2012
We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy.
