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JoVE Journal Biology
Detektion av protein interaktioner i Plant använder gateway Kompatibel komplettering Bimolecular Fluorescence (BiFC) System
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Detektion av protein interaktioner i Plant använder gateway Kompatibel komplettering Bimolecular Fluorescence (BiFC) System

Article DOI: 10.3791/3473 • 08:22 min
September 16th, 2011 •
1, 2, 2, 1, 2
1Department of Biology, University of Western Ontario, 2Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada

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September 16th, 2011

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Vi har utvecklat en teknik för att testa protein-protein interaktioner i anläggningen. En gul fluorescerande protein (YFP) delas upp i två icke-överlappande fragment. Varje fragment är klonade i-ram till en gen av intresse via gateway, vilket gör att uttryck av fusionsproteiner. Ombildning av YFP signalen bara uppstår när förhöret proteiner interagerar.

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