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JoVE Journal

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Skeletal Muscle Gender Dimorphism from Proteomics

Skeletal Muscle Gender Dimorphism from Proteomics

Article DOI: 10.3791/3536
December 14th, 2011


Summary December 14th, 2011

A straight-forward set of methods to isolate and determine the identity of the most abundant proteins expressed in skeletal muscle. About 800 spots are discerned on a two-dimensional gel from 10 mg muscle; this allows for the determination of gender-specific protein expression. These methods will give equivalent results in most tissues.

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