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Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays
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Chapters
- 00:05Title
- 01:23Dissection of the Dopaminergic Midbrain
- 03:03Dissection of the Striatum
- 04:04Assembly Collagen Matrix Assays
- 05:24Analysis by Immunohistochemistry and Quantification
- 07:27Results: Axon Growth in a Collagen Matrix Culture
- 08:00Conclusion
Explants from the midbrain dopamine system and striatum are used in a collagen matrix assay for the in vitro analysis of mesostriatal and striatonigral pathway development. In this assay axonal outgrowth and guidance can be manipulated and quantified. It can also be modified for assessing other regions or molecular cues.
Tags
DissectionCultureMouseDopaminergicStriatalExplantsThree-dimensional Collagen Matrix AssaysMidbrain Dopamine NeuronsStriatumAxon TractsAxon GrowthGuidance CuesSoluble FactorsIn VitroCollagen MatrixStable GradientsProteins ReleasedRat Tail Collagen PurificationMicrodissectionDopaminergic ExplantsStriatal ExplantsCollagen GelsImmunohistochemical AnalysisQuantitative AnalysisAxonal Projections
