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JoVE Journal
Immunology and Infection

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Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
 

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Article doi: 10.3791/3814
August 15th, 2012 Usage Statistics

Summary August 15th, 2012

Isolating primary microglia from the cellular heterogeneity of the brain is essential to investigate their role in both physiological and pathological conditions. This protocol describes a mechanical isolation and mixed cell culture technique that provides high yield and high purity, viable primary microglial cells for in vitro study and downstream applications.

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