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Obtaining Human Microglia from Adult Human Brain Tissue
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JoVE Journal Neuroscience
Obtaining Human Microglia from Adult Human Brain Tissue

Obtaining Human Microglia from Adult Human Brain Tissue

DOI:
10.3791/61438-v

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09:41 min

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August 30, 2020

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, , , ,
1Department of Bioscience and Bioengineering,Indian Institute of Technology Jodhpur, 2Department of Neurosurgery,All India Institute of Medical Sciences Jodhpur

Chapters

  • 00:00Introduction
  • 01:06Tissue Acquisition and Processing: Day 0
  • 05:35Cell Culture: Day 2
  • 07:59Cell Culture: Day 4
  • 08:26Results: Representative Images of Adult Human Microglia
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol is an efficient, cost effective and robust method of isolating primary microglia from live, adult, human brain tissue. Isolated primary human microglia can serve as a tool for studying cellular processes in homeostasis and disease.

Tags

Keywords: Human MicrogliaPrimary Microglia CellsImmunocytochemistryCell IsolationTrypsin-EDTACentrifugationCell Culture

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