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Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

DOI:
10.3791/3857-v

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07:46 min

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June 05, 2012

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, , , , ,
1Institute of Infection and Global Health,University of Liverpool , 2NIHR Biomedical Research Centre in Microbial Disease,University of Liverpool

Chapters

  • 00:05Title
  • 01:24Preparation of Artificial Sputum Medium (ASM) for Biofilm Cultures
  • 03:14Determination of Biofilm Sessile Cell Minimum Inhibitory Concentration (BSMIC)
  • 06:22Results: Properties and Antibacterial Resistance of P. aeruginosa Biofilms or Planktonic Cultures
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

Current diagnostic antimicrobial susceptibility testing relies on the planktonic growth of isolates in nutrient rich, aerobic conditions. Here, we employ an alternative artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilms under both aerobic and microaerophilic conditions more representative of the cystic fibrosis lung.

Tags

Artificial Sputum MediumAntibiotic EfficacyPseudomonas AeruginosaCystic Fibrosis LungIn Vitro Antimicrobial Susceptibility TestsMicroaerophilic ConditionsBiofilmsArtificial Sputum Medium AssayMicrotitre-plate AssayAntimicrobial Susceptibility

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