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JoVE Journal
Immunology and Infection

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Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
 

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Article doi: 10.3791/56430
August 3rd, 2017

Summary August 3rd, 2017

A simple protocol for overexpression and purification of codon-optimized, human cis-prenyltransferase, under non-denaturing conditions, from Escherichia coli, is described, along with an enzymatic activity assay. This protocol can be generalized for production of other cis- prenyltransferase proteins in quantity and quality suitable for mechanistic studies.

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