Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
 

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Article DOI: 10.3791/56430
August 3rd, 2017

Chapters

Summary August 3rd, 2017

A simple protocol for overexpression and purification of codon-optimized, human cis-prenyltransferase, under non-denaturing conditions, from Escherichia coli, is described, along with an enzymatic activity assay. This protocol can be generalized for production of other cis- prenyltransferase proteins in quantity and quality suitable for mechanistic studies.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter