Name: Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET (Video) | JoVE Journal
Uploaded: 2013-04-28T14:00:00.000+00:00
Duration: 17 min 13 s
Description: Learn non-invasive PET imaging for acute allograft rejection assessment using a rat renal transplantation model. This method enables metabolic monitoring of transplant health with 18F-FDG.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

This work was supported by the Deutsche Forschungsgemeinschaft (SFB 656, M&uuml;nster, Germany, projects C7 &amp; C6) and the IZKF M&uuml;nster (Core unit SmAP). The authors are grateful to Truc Van Le, Anne Kanzog, Ute Neugebauer, Wiebke Gottschlich and Roman Priebe for excellent technical assistance and to Daniel Burkert and Sven Fatum for producing radiotracers.

1. Donor Organ Recovery
<ol>
 <li>Set up the stereotactic microscope, record weight of the 8-10 week old rat (donor and recipient body weight should match).</li>
 <li>Anesthetize the donor rat (Lewis Brown Norway F1, LBN F1) using oxygen/isoflurane inhalation (isoflurane 4 %/2 L/min oxygen). Maintain anesthesia by lowering isoflurane to 2-2.5%.</li>
 <li>Place the anesthetized rat on the surgery pad. Fix the rat's extremities with adhesive tape onto the pad and apply ophthalmic ointment (Bepanthen, Bayer) to the rat's eyes.</li>
 <li>Shave and disinfect the abdomen of the rat and perform a ventral midline incision from the pubis to the caudal border of the liver. Expose the left kidney and its vessels by carefully moving the intestine to the right side. Place a thin sheet of gauze over the left kidney and moisten it with warmed isotonic saline solution to prevent desiccation.</li>
 <li>Carefully dissect the fat tissue with Dumont Angled Tip Forceps (Dumont SS-45 Forceps Inox Medical, Fine Science Tools, cat.no. 11203-25) enveloping the left ureter. Avoid any direct contact with the ureter, instead, mobilize it with sufficient surrounding fat tissue.</li>
 <li>Separate the left renal vein from the renal artery using Dumont Angled Tip forceps. Remove all fat and connective tissue from both vessels and cauterize the adrenal and testicular vessels.</li>
 <li>Dissect aorta and inferior vena cava (IVC) above and below its junction with the left renal artery and vein, respectively, and cauterize all outgoing arteries.</li>
 <li>Clamp the suprarenal aorta with a microsurgical clamp (Micro Serrefines, Fine Science Tools, cat.no. 18055-04) above the left renal artery, as near as possible to the superior mesenteric artery. Ligate the infrarenal aorta using surgical silk (5-0, V&ouml;mel, art no. 14739) approximately 5 mm below the left renal artery.</li>
 <li>Ligate the infrarenal IVC with surgical silk (5-0, V&ouml;mel), and clamp the suprarenal IVC with a microsurgical clamp (Micro Serrefines, Fine Science Tools, cat.no. 18055-03).</li>
 <li>Cut the renal vein as close as possible to the IVC using fine scissors (Iris Scissors - ToughCut Straight 11.5 cm, Fine Science Tools, cat.no. 14058-11).</li>
 <li>Slowly perfuse the kidney in situ with 2 ml ice-cold HTK perfusion solution (CUSTODIOL HTK, Dr. Franz K&ouml;hler Chemie) by inserting a canula (Microlance 3 27G &frac34;, BD, cat.no. 302200) in the infrarenal aorta. Confirm that the renal color changes (now olive-tinted).</li>
 <li>First, transect the suprarenal aorta, then the infrarenal aorta and finally the ureter as close as possible to the urinary bladder.</li>
 <li>Remove the kidney and its vascular supply together with the ureter and store in HTK perfusion solution on ice.</li>
 <li>Euthanize the donor rat by removing the vascular clamps and consecutive excision of the heart under anesthesia.</li>
</ol>
2. Recipient Preparation and Transplantation
<ol>
 <li>Set up the stereotactic microscope, record weight of the rat.</li>
 <li>Anesthetize the recipient rat (Lewis) using isoflurane 4%. Maintain anesthesia by lowering isoflurane to 2-2.5%.</li>
 <li>Place the anesthetized rat on the surgery pad. Fix the rat's extremities with adhesive tape onto the pad and apply ophthalmic ointment to the rat`s eyes. Monitor and control core body temperature using a rectal temperature sensor and a warming pad. During surgery repetitively control temperature, breathing and heart rate of the animal.</li>
 <li>Shave and disinfect the abdomen and perform a ventral midline incision from the pubis to the caudal border of the liver. Use a scalpel forcutting the skin in order to minimize skin trauma. Expose the left kidney and its vessels by moving the intestine to the right side.</li>
 <li>Carefully remove the renal capsule using Dumont Angled Tip forceps and cotton swabs.</li>
 <li>Occlude the left renal artery, vein and ureter near the renal hilum with two ligations using surgical thread (Mersilene 4-0, Ethicon, cat. no. EH6732H). Excise the greater part of the kidney, leaving only the renal hilum. Clean the cut surface with cotton swabs and check for bleeding. Add another ligature if necessary.</li>
 <li>Carefully dissect bluntly through the connective tissue covering the infrarenal aorta and vena cava on the caudal site with cotton swabs.</li>
 <li>Separate any visible nerve from the infrarenal aorta and vena cava using Dumont Angled Tip forceps.</li>
 <li>Carefully dissect through the connective tissue sheet between the infrarenal aorta and vena cava, forming two openings of 2-4 mm length: The first below the branching of the testicular vessels, and the second one above the branching of the common iliac vessels. Cauterize the iliolumbar arteries and veins in between.</li>
 <li>Draw a single surgical thread (Mersilene 0; Ethicon, cat. no.EH6665E) through each of the two openings. Using these threads pull the infrarenal aorta and vena cava gently to the right side of the animal to gain access to the vessels branching on the dorsal site. Cauterize any of these vessels between the two openings and remove the surgical threads thereafter.</li>
 <li>Next, occlude aorta and IVC by clockwise application of four microsurgical clamps (Micro Serrefines, Fine Science Tools, cat.no. 18055-04) beginning at the upper opening with the aorta and ending with the vena cava at the upper opening (Micro Serrefines, Fine Science Tools, cat.no. 18055-03).</li>
 <li>Afterwards carefully pierce the upper ending of the isolated part of the aorta with a needle (Microlance 3 27G &frac34;, BD, cat.no. 302200), insert the lower blade of a fine spring scissor (Vannas Spring Scissors - 3 mm Blades, Fine Science Tools, cat.no 15000-00) through the puncture opening and perform a short longitudinal incision. Flush the isolated aorta with ice-cold HTK perfusion solution to remove any remaining blood.</li>
 <li>Likewise, carefully pierce the lower ending of the isolated part of the vena cava with a needle, insert the lower blade of a fine spring scissor through the puncture opening and perform a longitudinal incision ending shortly below the aortic incision. Again, flush the vessel to remove any remaining blood.</li>
 <li>Remove the kidney graft from the storage solution. Place it below the former position of the left kidney, and ensure that it is correctly oriented.</li>
 <li>Check the orientation of the grafts renal artery and ensure that there are no twists. Then place a thin sheet of gauze over the graft and moisten it with ice-cold HTK perfusion solution to cool it, and to prevent desiccation.</li>
 <li>Fixate the opening of the suprarenal part of the aortic piece in which the renal artery of the graft opens into with surgical thread (Ethilon 9-0, Ethicon, cat. no. 2809G) - first at the upper, then at the lower end of the aortic incision. Close the incision clockwise with a continuous suture using surgical thread (Ethilon 9-0), Dumont Angled Tip forceps and a curved Castroviejo needle holder (Fine Science Tools, cat. no. 12061-01). Afterwards, cover the first suture with connective tissue through a counterclockwise second continuous suture. Now, only the lower part of the aortic piece of the graft remains to be closed. Regularly cool the graft with ice-cold HTK perfusion solution.</li>
 <li>Perfuse the graft with 1 ml of ice-cold HTK perfusion solution through a blunt needle inserted into the open end of the grafts aortic piece validating the tightness and integrity of the suture, as well as removing remaining blood in the graft. Afterwards, ligate the open end of the aortic piece with surgical silk (5-0, V&ouml;mel).</li>
 <li>Check the orientation of the grafts renal vein and ensure that there are no twists.</li>
 <li>Fixate the opening of the vein of the graft with surgical thread (Ethilon 9-0) - first at the upper, then at the lower end of the incision at the vena cava. Close the incision clockwise with a continuous suture using surgical thread (Ethilon 9-0), Dumont Angled Tip forceps and a curved Castroviejo needle holder. Regularly cool the graft with ice-cold HTK perfusion solution.</li>
 <li>Counterclockwise remove the microsurgical clamps, beginning with the uppermost vena cava clamp. After removing the last aortic clamp, carefully stop eventually mild bleeding with cotton swabs. After a few seconds the graft will turn rosy-tinted, and uretric contractions should appear.</li>
 <li>For the insertion of the grafts ureter into the recipient`s bladder carefully remove a small patch of the musculature at the top of the bladder using a Vannas Spring Scissor (Student Vannas Spring Scissor straight, Fine Science Tools, cat.no. 91500-09). Then, remove the surrounding tissue (mostly fat) from the graft ureter using Dumont Angled Tip forceps. Therefore, carefully grab the tip of the ureter and gently separate the surrounding fat, connective tissue and the embedded vessels from it by pulling them in the opposite direction. Fixate the uretric tip at the end of a surgical thread (Prolene 6-0, Ethicon, cat. no. 8697H) and perforate the previously prepared top of the bladder with the attached needle. Pull the ureter through the bladder and exit it at the base through a second perforation.</li>
 <li>Fixate the previously from the ureter separated tissue at the top of the bladder with surgical thread (Ethilon 9-0). Cut off the tip of the ureter with the attached surgical thread and pull the ureter back into the bladder by gently pushing from below. Afterwards, close the second bladder puncture with surgical thread (Ethilon 9-0).</li>
 <li>Close the abdominal wall and skin by two continuous but separate sutures using surgical suture thread (Mersilene 0). Disinfect the wound using Pividon Iodine and administer buprenorphine (0.1 mg per kg/BW/bid) s.c. for three days after surgery to control wound pain.</li>
</ol>
3. Imaging Allograft Rejection
<ol>
 <li>Anesthetize nonfasting rat using isoflurane 2-2.5%.</li>
 <li>30 MBq FDG (30 MBq FDG in 0.1 ml 0.9% NaCl) are administered i.v. via catheterizing a lateral tail vein using a 24 G catheter (Braun, Introcan, 4252500-01). Afterwards, purge the catheter with 0.9 ml 0.9% NaCl solution.</li>
 <li>Leave the rat in a restrainer without anesthesia until start of the scan and hydrate the animal by i.v. injection of 1 ml of 0.9% NaCl solution hourly.</li>
 <li>Re-anesthetize rat using isoflurane 2% immediately before the scan starts.</li>
 <li>Place anesthetized rat in a high-resolution multi-wire chamber-based animal PET camera quadHIDAC (Oxford Positron Systems Ltd, Oxford, UK). The spatial resolution of the PET scanner is 1.0 mm and is constant over the whole FOV (diameter, 165 mm; axial length, 280 mm). Monitor and control body temperature during the scan using a rectal temperature sensor and control anesthesia with a pulse oxymeter.</li>
 <li>Start dynamic acquisition for 60 min starting 180 min after FDG-injection to reduce tracer accumulation in the kidneys caused by renal excretion of FDG.</li>
 <li>Apply 5 MBq of 18F-fluoride i.v. and perform another PET scan immediately after 18F-fluoride injection for 60 min without moving the position of the rat in the scanner for identification of renal parenchyma and for calculation of 18F-clearance 5. Reconstruct images from both scans. Trace manually a volume of interest (VOI) on reconstructed images 2 min after 18F-fluoride injection (perfusion-phase) around renal cortex and transfer the VOI to the FDG images. Carefully exclude the renal pelvis from the VOI. Reconstruct images from both scans and manually trace a volume of interest (VOI) around renal cortex. List-mode data were reconstructed into images with a voxel size of 0.4 &times; 0.4 &times; 0.4 mm3. Carefully exclude the renal pelvis from the VOI.</li>
 <li>Compute FDG uptake by the ratio of total counts and volume and calculate the percentage of injected dose (%ID). We used MATLAB (Version R2011b, MathWorks, Natick, MA, USA) and tomographic imaging (TIM) Version 2.8 for image analysis.</li>
</ol>

<ol><li>Wu, O., Levy, A. R., Briggs, A., Lewis, G., Jardine, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+rejection+and+chronic+nephropathy%3A+a+systematic+review+of+the+literature%5BTitle%5D)+AND+%22Transplantation%22%5BJournal%5D)">Acute rejection and chronic nephropathy: a systematic review of the literature.</a> Transplantation. 87, 1330-1339 (2009).</li>
<li>Miller, B. F., Gonzales, E., Wilchins, L. J., Nathan, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Kidney+transplantation+in+the+rat%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Kidney transplantation in the rat.</a> Nature. 194, 309-310 (1962).</li>
<li>Reuter, S., Schnöckel, U., Schröter, R., Schober, O., Pavenstädt, H., Schäfers, M., Gabriëls, G., Schlatter, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-invasive+imaging+of+acute+renal+allograft+rejection+in+rats+using+small+animal+F-FDG-PET%5BTitle%5D)+AND+%22PLoS.+One%22%5BJournal%5D)">Non-invasive imaging of acute renal allograft rejection in rats using small animal F-FDG-PET.</a> PLoS. One. 4, e5296(2009).</li>
<li>Reuter, S., Schnöckel, U., Edemir, B., Schröter, R., Kentrup, D., Pavenstädt, H., Schober, O., Schlatter, E., Gabriëls, G., Schäfers, M. Potential of noninvasive serial assessment of acute renal allograft rejection by 18F-FDG PET to monitor treatment efficiency. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aReuter%2C+S+%22J.+Nucl.+Med%22">J. Nucl. Med</a>. 51, 1644-1652 (2010).</li>
<li>Schnöckel, U., Reuter, S., Stegger, L., Schlatter, E., Schäfers, K. P., Hermann, S., Schober, O., Gabriëls, G., Schäfers, M. Dynamic 18F-fluoride small animal PET to noninvasively assess renal function in rats. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSchn%C3%B6ckel%2C+U+%22Eur.+J.+Nucl.+Med.+Mol.+Imaging%22">Eur. J. Nucl. Med. Mol. Imaging</a>. 35, 2267-2274 (2008).</li>
<li>Grabner, A., Schnöckel, U., Kentrup, D., Schäfers, M., Reuter, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Strategies+for+Non-Invasive+Molecular+Imaging+of+Acute+Allograft+Rejection+by+Gamma+Scintigraphy+and+Positron+Emission+Tomography%5BTitle%5D)+AND+%22Curr.+Radiopharm%22%5BJournal%5D)">Strategies for Non-Invasive Molecular Imaging of Acute Allograft Rejection by Gamma Scintigraphy and Positron Emission Tomography.</a> Curr. Radiopharm. 4, 10-23 (2011).</li>
<li>Racusen, L. C., Solez, K., Colvin, R. B., Bonsib, S. M., Castro, M. C., Cavallo, T., Croker, B. P., Demetris, A. J., Drachenberg, C. B., Fogo, A. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+Banff+97+working+classification+of+renal+allograft+pathology%5BTitle%5D)+AND+%22Kidney+Int%22%5BJournal%5D)">The Banff 97 working classification of renal allograft pathology.</a> Kidney Int. 55, 713-723 (1999).</li>
<li>Edemir, B., Reuter, S., Borgulya, R., Schröter, R., Neugebauer, U., Gabriëls, G., Schlatter, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+rejection+modulates+gene+expression+in+the+collecting+duct%5BTitle%5D)+AND+%22J.+Am.+Soc.+Nephrol%22%5BJournal%5D)">Acute rejection modulates gene expression in the collecting duct.</a> J. Am. Soc. Nephrol. 19, 538-546 (2008).</li>
<li>Velic, A., Gabriëls, G., Hirsch, J. R., Schröter, R., Edemir, B., Paasche, S., Schlatter, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+rejection+after+rat+renal+transplantation+leads+to+downregulation+of+Na%2B+and+water+channels+in+the+collecting+duct%5BTitle%5D)+AND+%22Am.+J.+Transplant%22%5BJournal%5D)">Acute rejection after rat renal transplantation leads to downregulation of Na+ and water channels in the collecting duct.</a> Am. J. Transplant. 5, 1276-1285 (2005).</li>
<li>Reuter, S., Velic, A., Edemir, B., Schröter, R., Pavenstädt, H., Gabriëls, G., Bleich, M., Schlatter, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protective+role+of+NHE-3+inhibition+in+rat+renal+transplantation+undergoing+acute+rejection%5BTitle%5D)+AND+%22Pflugers+Arch%22%5BJournal%5D)">Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection.</a> Pflugers Arch. 456, 1075-1084 (2008).</li>
</ol>

No conflicts of interest declared.

FDG-PET imaging is a new option for the diagnosis of acute rejection. Because of its non-invasive and specific nature, FDG-PET is advantages in comparison to classical diagnostics by core needle biopsy. In contrast to the limited sample size of a biopsy, FDG-PET analysis the whole graft. Moreover, one can apply it to patients on anticoagulant therapy, and one can perform PET measures repetitively e.g. to monitor treatment efficiency 4. In addition, we already have shown that two major different...

<table><tbody><tr><td colspan="4">&lt;strong&gt;Equipment&lt;/strong&gt;</td></tr><tr><td>Mathieu Needle Holder - 14 cm</td><td>Fine Science Tools</td><td>12010-14</td><td></td></tr><tr><td>Castroviejo Micro Needle Holder - 9 cm</td><td>Fine Science Tools</td><td>12061-01</td><td></td></tr><tr><td>Surgical Scissors - Sharp_Blunt</td><td>Fine Science Tools</td><td>14001-12</td><td></td></tr><tr><td>Iris Scissors - ToughCut Straight 11.5 cm</td><td>Fine Science Tools</td><td>14058-11</td><td></td></tr><tr><td>Student Vannas Spring Scissors </td><td>Fine Science Tools</td><td>91500-09</td><td></td></tr><tr><td>Vannas Spring Scissors - 3 mm Blades</td><td>Fine Science Tools</td><td>15000-00</td><td></td></tr><tr><td>Student Tissue Forceps - 1x2 Teeth 12 cm</td><td>Fine Science Tools</td><td>91121-12</td><td></td></tr><tr><td>Dumont SS-45 Forceps - Inox Medical</td><td>Fine Science Tools</td><td>11203-25</td><td></td></tr><tr><td>Micro-Serrefine Clip Applicator with Lock</td><td>Fine Science Tools</td><td>18056-14</td><td></td></tr><tr><td>Micro-Serrefine 6 mm x 1 mm</td><td>Fine Science Tools</td><td>18055-03</td><td></td></tr><tr><td>Micro-Serrefine 4 mm x 0.75 mm</td><td>Fine Science Tools</td><td>18055-04</td><td></td></tr><tr><td colspan="4">&lt;strong&gt;Reagent&lt;/strong&gt;</td></tr><tr><td>Isoflurane (&lt;em&gt;e.g.&lt;/em&gt; Forene 100% v/v)</td><td>Abott</td><td></td><td></td></tr><tr><td>cutane antiseptic (&lt;em&gt;e.g.&lt;/em&gt; Octeniderm) </td><td>Sch&amp;#252;lke</td><td></td><td></td></tr><tr><td>Povidone Iodine (&lt;em&gt;e.g.&lt;/em&gt; Betaisodona)</td><td>Mundipharma</td><td></td><td></td></tr><tr><td>ophthalmic ointment (&lt;em&gt;e.g.&lt;/em&gt; Bepanthen)</td><td>Bayer</td><td></td><td></td></tr><tr><td>Buprenorphin (&lt;em&gt;e.g.&lt;/em&gt; Temgesic)</td><td>RB Pharmaceuticals</td><td></td><td></td></tr><tr><td>HTK perfusion solution (&lt;em&gt;e.g.&lt;/em&gt; CUSTODIOL HTK)</td><td>Dr. Franz K&amp;#246;hler Chemie</td><td></td><td></td></tr><tr><td>surgical thread Mersilene 0</td><td>Ethicon</td><td>EH6665E</td><td></td></tr><tr><td>surgical thread Mersilene 4-0</td><td>Ethicon</td><td>EH6732H</td><td></td></tr><tr><td>surgical thread Prolene 6-0</td><td>Ethicon</td><td>8697H</td><td></td></tr><tr><td>surgical thread Ethilon 9-0</td><td>Ethicon</td><td>2809G</td><td></td></tr><tr><td>surgical silk 5-0</td><td>V&amp;#246;mel</td><td>14739</td><td></td></tr><tr><td>Canula (&lt;em&gt;e.g.&lt;/em&gt; Microlance 3, 27G &amp;#190;)</td><td>BD</td><td>302200</td><td></td></tr></tbody></table>

non-invasive imaging of acute allograft rejection after rat renal transplantation using 18f-fdg pet

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

The number of patients with end-stage renal disease, and the number of kidney allograft recipients continuously increases. Episodes of acute cellular allograft rejection (AR) are a negative prognostic factor for long-term allograft survival, and its timely diagnosis is crucial for allograft function 1. At present, AR can only be definitely diagnosed by core-needle biopsy, which, as an invasive method, bares significant risk of graft injury or even loss. Moreover, biopsies are not feasible in patients taking anticoagulant drugs and the limited sampling site of this technique may result in false negative results if the AR is focal or patchy. As a consequence, this gave rise to an ongoing search for new AR detection methods, which often has to be done in animals including the use of various transplantation models.
Since the early 60s rat renal transplantation is a well-established experimental method for the examination and analysis of AR 2. We herein present in addition small animal positron emission tomography (PET) using 18F-fluorodeoxyglucose (FDG) to assess AR in an allogeneic uninephrectomized rat renal transplantation model and propose graft FDG-PET imaging as a new option for a non-invasive, specific and early diagnosis of AR also for the human situation 3. Further, this method can be applied for follow-up to improve monitoring of transplant rejection 4.

Histology
During AR leukocytes, i.e. mainly T-lymphocytes are recruited into the transplant, whereas the severity of the rejection is reflected by the degree of inflammation. In the Periodic-Acid-Schiff (PAS) staining depicted here (Figure 1), the renal allograft shows significant histological signs of AR, namely glomerulitis, tubulitis, endothelialitis and graft infiltration (Figure 1, aTX POD4) (POD = postoperative day) while signs of rejection a...

We herein present a rat renal transplantation model to non-invasively assess acute allograft rejection using positron emission tomography with 18F-fluorodeoxyglucose.

The number of patients with end-stage renal  disease, and the number of kidney allograft recipients continuously increases.  Episodes of acute cellular ...

non-invasive-imaging-acute-allograft-rejection-after-rat-renal

Research

JoVE Journal

Medicine

This article describes multimodality imaging of cancerous tissues with PET and CT diagnostic imaging by using a radiotracer 18F-FDG. Cancerous tissue with high glycolytic activity uptakes more 18F-FDG.

FDG Based PET/CT Scan: A Method of Using Radiotracer for Non-invasive Imaging of Tumor Metabolism

Brain damage resulting from cerebral ischemia may be non-invasively imaged and studied in rats using pre-clinical positron emission tomography coupled with the injectable radioactive probe, 18F-fluorodeoxyglucose. Further, the use of modern software tools that include volume of interest (VOI) brain templates dramatically increase the quantitative information gleaned from these studies.

Non-invasive Imaging and Analysis of Cerebral Ischemia in Living Rats Using Positron Emission Tomography with 18F-FDG

Here, we present a protocol to study the immunology of rejection. The surgical model presented reports a short operating time and a concise technique. Depending on the donor-recipient strain combination, the transplanted kidney may develop acute cellular rejection or chronic allograft damage, defined by interstitial fibrosis and tubular atrophy.

Mouse Kidney Transplantation: Models of Allograft Rejection

The bioorthogonal inverse electron demand Diels-Alder cycloaddition has been harnessed to create an effective and modular pretargeted PET imaging strategy for cancer. In this protocol, the steps of this methodology are described in the context of a model system employing the colorectal cancer targeted antibody huA33 and a 64Cu-labeled radioligand.

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

The αvβ3 integrin is a type of adhesion protein that is highly expressed on activated endothelial cells undergoing angiogenesis. Thus, evaluating the integrity of the integrin is of great interest in oncology. Here, we introduce a method to prepare 68Ga-labeled radiopeptides and a method to assess its biological effectiveness.

Preparing a 68Ga-labeled Arginine Glycine Aspartate (RGD)-peptide for Angiogenesis

The purpose of this manuscript and protocol is to explain and demonstrate in detail the surgical procedure of orthotopic kidney transplantation in rats. This method is simplified to achieve the correct perfusion of the donor kidney and shorten the reperfusion time by using the venous and ureteral cuff anastomosis technique.

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection

Here, we present a protocol to create orthotopic hepatocellular carcinoma xenografts with and without hepatic artery ligation and perform non-invasive positron emission tomography (PET) imaging of tumor hypoxia using [18F]Fluoromisonidazole ([18F]FMISO) and [18F]Fluorodeoxyglucose ([18F]FDG).

Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

Here, we present an experimental imaging protocol for the quantification of cardiac function and morphology using high-resolution positron emission tomography/computed tomography for small animals. Both mice and rats are considered, discussing the different requirements of computed tomography contrast agents for the two species.

High-Resolution Cardiac Positron Emission Tomography/Computed Tomography for Small Animals

Here, we present a protocol to utilize [68Ga]-NOTAFAP-2286 PET/CT imaging to exhibit high sensitivity in detecting and precisely localizing tendon injuries.

PET/CT With [68Ga]-NOTA-FAP-2286 for Imaging of Tendon Injuries in Rat Achilles Tendon Injury Models

Learn non-invasive PET imaging for acute allograft rejection assessment using a rat renal transplantation model. This method enables metabolic monitoring of transplant health with 18F-FDG.

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using ¹⁸F-FDG PET

Explore More Videos