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JoVE Journal
Neuroscience

This content is Open Access.

Neuromodulation and Mitochondrial Transport
 

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Article doi: 10.3791/2599
June 17th, 2011

Summary June 17th, 2011

We describe a protocol that allows imaging of mitochondria in living neurons via fluorescence microscopy over long durations. Imaging over extended periods is accomplished through lentivirus-mediated expression of a mitochondrially targeted fluorescent protein and use of an inexpensive stage-top incubator that was designed and built in our laboratory.

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