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Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

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04:50 min

June 17, 2011

DOI:

04:50 min
June 17, 2011

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Summary

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We describe a protocol that allows imaging of mitochondria in living neurons via fluorescence microscopy over long durations. Imaging over extended periods is accomplished through lentivirus-mediated expression of a mitochondrially targeted fluorescent protein and use of an inexpensive stage-top incubator that was designed and built in our laboratory.

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