Journal
/
Neuroscience
/
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

DOI:
10.3791/2599-v

•

04:50 min

•

June 17, 2011

•

, ,
1Department of Experimental Neurobiology,The Neurosciences Institute

Chapters

  • 00:05Title
  • 01:17Overview of Stage-top Incubator for Long-term Neuronal Imaging
  • 01:46Application of the Membrane Lid to the Culture Dish and Placement in the Incubator
  • 02:19Live Imaging of Mitochondria in Hippocampal Neurons
  • 03:44The Effects of Neuromodulation on Mitochondrial Movement in Cultured Hippocampal Neurons
  • 04:27Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol that allows imaging of mitochondria in living neurons via fluorescence microscopy over long durations. Imaging over extended periods is accomplished through lentivirus-mediated expression of a mitochondrially targeted fluorescent protein and use of an inexpensive stage-top incubator that was designed and built in our laboratory.

Tags

NeuromodulationMitochondrial TransportLive ImagingHippocampal NeuronsExtended DurationsVital DyesFluorescent ProteinsCytoskeletal ComponentsOrganellesDynamic Fluorescence MicroscopyRhodamine 123Mitochondrially Targeted EYFPPrimary NeuronsCulture ConditionsTemperatureHumidityPHFluorescent SignalSignal IntensityImage AnalysisExposure TimesPhotobleachingPhototoxicity

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2599?article_type=v

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
check_url/2599?article_type=v

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
check_url/2599?article_type=v

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
check_url/2599?article_type=v

Calcium Phosphate Transfection of Primary Hippocampal Neurons
check_url/2599?article_type=v

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
check_url/2599?article_type=v

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
check_url/2599?article_type=v

Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons
check_url/2599?article_type=v

Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons
check_url/2599?article_type=v

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
check_url/2599?article_type=v

Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons

Read Article