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Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities
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Neuroscience
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JoVE Journal Neuroscience
Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities

Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities

DOI:
10.3791/50557-v

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08:10 min

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March 31, 2014

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, , , ,
1Department of Molecular Physiology & Biophysics,University of Iowa Carver College of Medicine, 2Department of Biology & Biochemistry,University of Bath

Chapters

  • 00:05Title
  • 02:02Staining Based on Spontaneous Synaptic Activity
  • 03:38Washing Out the FM Dye and Searching for Optimal Image Field
  • 04:56Unloading FM Dye from the Synaptic Vesicles
  • 06:19Results: Representative Result of Destaining
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the use of styryl FM dyes to image synaptic vesicle recycling in functional nerve terminals. This protocol can be applied not only to evoked, but also spontaneous and miniature synaptic activities. The protocol expands the variety of synaptic events that can be effectively evaluated.

Tags

Keywords: Synaptic Vesicle RecyclingFM DyesEvoked Synaptic ActivitySpontaneous Synaptic ActivityMiniature Synaptic ActivityMembrane TurnoverHippocampal NeuronsPhotobleaching

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